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mutant mice. These F1 heterozygous mice were backcrossed with 129S5 mice 1–two times just before getting used for intercrossing. Genotyping PCR consisted of a 540 bp product or service amplified with the wild-sort (wt) allele using a forward primer A (CAACTGTCTTCTCATTAGTGG) inside the wt sequence deleted by targeted mutation along with a reverse

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